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Join Valitacell at Cell Line Development & Engineering Asia and find out about Clone Stability Assessment & Rapid Antibody Titer Quantification.
 

Podium Presentation: Generation of Unique Clone Fingerprints for Control and Enhancement of CHO Cell Factories
Wednesday, 16 May | 12.00

Presentation by: Dr Carolanne Doherty, Valitacell
The utility of CHO cell factories derives from exploitation of their acquired genetic/functional variation, which enable industry to identify cell lineages with desirable manufacturing properties. Here we discuss novel technologies engineered to provide process control while at the same time enabling optimal leverage of the cell factory using ChemStress fingerprinting. This includes a novel high-throughput assay for the simple, effective antibody titer measurement.

Podium Presentation:  Liquid Engineering: CHO Cell Culture Performance Enhancement Using Small Molecules
Thursday, 17 May | 9.10

Presentation by: Prof. David James, University of Sheffield

In contrast to genetic engineering of CHO cells to enhance their functional performance, bioactive small molecule (SM) additives are relatively simple to utilise. They can be screened, titrated, combined and deployed during culture with comparative ease. We describe the development of a medium to high-throughput platform that enables rapid quantitative assessment of SM additives as a tool to create bespoke media environments designed to engineer cell factory function for optimal manufacturing performance.

Poster Presentation: Liquid Engineering: CHO cell clone stability assessment using functional phenotype array profiling
Presentation by: Dr. Jerry Clifford, Valitacell

Instability of recombinant protein production from CHO cell lines remains a serious bioprocess concern. Stability is loosely defined as a loss of titer over a defined period of time (typically >60 generations). There is no consensus definition of an “unstable” cell line in industry, nor are there clear regulatory guideline on what constitutes a stable cell line. Many companies find that a substantial proportion of their candidate clones fail due to instability problems, having initially demonstrated good production performance in small-scale bioreactors. Using the ChemStress fingerprinting Approach approach, we demonstrated that by measuring changes in chemical stress response over time it was possible to differentiate stable and unstable producing cell lines using a simple LDA modeling approach. It is predicted that this phenotypic stress response stability screening coupled with our modeling approach could potentially shorten and simplify stability screening studies. Using a simple model, an 85% separation success could be achieved which correctly classified 27/32 clones as either stable or unstable.

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